Antimicrobial properties of Theruptor 3D-hydrocellular wound dressing: An in vitro study

ABSTRACT Background: Colonized wound infections become a major public health concern. Infections may lead to delayed healing process or severe complications. Thus, the incorporation of antimicrobial agents such as silver, iodine, etc., into the dressing material provides protection against microbes. However, these agents have limited functional usage. Recently, Dimethyl tetradecyl [3-(trimethoxysilyl)propyl] ammonium chloride (DTAC) based 3D-hydrocellular wound dressings have emerged. Therefore, we evaluated the short- and long-term antibacterial efficacy of Theruptor 3D-hydrocellular wound dressing. Methods: The antimicrobial activity of the dressing was evaluated using modified method of AATCC100. Inoculums of 27 differential microbes including gram-positive bacteria, gram-negative bacteria, and fungus were obtained. The fabric samples were inoculated with a different microbial sample in a sterile petri plate. The samples were incubated for short (1 min, 30 min, 1h, and 4h) and long (7, 14, and 28 days) term durations. Results: Initially, the growth of test microorganisms ranged between 7.9 × 10 6 to 2.38 × 10 7 CFU/ml at 0 min. After respective time intervals, the growth of all the tested microbes was significantly reduced in a time-dependent manner ( p < 0.05). The dressing material achieved zero CFU/ml at 4 h. Moreover, it showed a significant reduction of >5 log at 1 h till 28 days. Conclusion: Based on the “physical kill mechanism”, Theruptor 3D-hydrocellular wound dressing not only provides protection against a broad spectrum of pathogens but for a wide-ranging time period i.e., 1 min to 28 days that ensures effective and significant wound healing. Thus, it may consider as a promising advancement in the wound care settings. Highlights