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Noise pollution poses a serious threat to public health and continues to grow in extent, frequency, and severity due to the rapid population growth and urbanization, and this is of particular concern in developing countries such as Rwanda. However, data on noise pollution levels, noise laws and regulations are, however, lacking in Rwanda. We assessed the effect of land-use type during a two-month period at nine sites: three commercial sites, three passenger-car parking sites, two road junction sites, and one reference site (Car-Free Zone) in Rwanda. We collected data on noise pollution during weekdays (Monday, Wednesday, and Friday) and Weekends (Saturday and Sunday) in the morning (7h00-10h00), around noon (11h00-14h00), and in the evening (15h00-18h00). The mean noise levels were higher during weekdays (60-80) A-weighted decibels (dB) (A)) than during weekends (50-70 dB (A)). We recorded the lowest noise level at Kigali car-free zone in the morning (34.4 dB (A)) and the highest noise level at Nyabugogo passenger-car parks in the evening (111.2dB (A)). Spatial variation of noise levels interpolated for Kigali City shows higher noise levels (hotspot) in the outskirts of Kigali, Remera and Kimironko. Noise levels recorded in Kigali exceeded the World Health Organization permissible daytime limits during both weekdays and weekends at all land-use types except the car-free zone site. Our results indicate that Kigali residents are exposed to high levels of noise, and urgent development of noise pollution monitoring programs and control measures in Rwanda is required.