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Income poverty remains the main root of urban food insecurity as purchased food represents a major component of the spending of urban residents. The rapid and increasing urbanization and urban sprawl are major concerns for food security in Mali. In Mali, 3.6% of households, are severely food insecure and the food insecurity prevalence rate for urban households is 7.5%. The effect of urbanization on households’ food security was assessed in the literature using only a single measure of food security. In this regard, this study analyzed the effect of urbanization on both objective and subjective measures of food security in Mali, using data from the national survey on food security and nutrition (ENSAN). The data collection was conducted by the Early Warning System against Famine (SAP) on 9,782 households in February 2018. For the objective measure of food security, the study used an OLS model to estimate the effect of urbanization on household food expenditure per capita. Using the household hunger scale (HHS) score, the study estimated two models (logit and ordered probit) to capture the effect of urbanization on subjective measures of food security. The results of the OLS and logit models showed that households living in urban areas are less food secure compared to their counterparts living in rural areas. The results from the ordered probit also confirmed that urban households are more food insecure (mildly and moderately) compared to their counterparts living in rural areas. Based on these results, the study recommends to decision-makers to take into account urban poor households when setting up food safety net programmes.