198 Differential Gene Expression and Metabolic Pathway Comparisons of Liver and Duodenum from Suckling Piglets Given One or Two Iron Dextran Injections

Abstract Six female littermate pairs were used in an experiment to evaluate the mRNA expression in tissues from piglets given one or two 1 mL injections of iron dextran (200 mg Fe/mL, Uniferon, Pharmacosmos Inc. Watchung, NJ). All pigs in the litter were administered the first 1 mL injection < 24 hours after birth and on d 7, pigs were paired by body weight (BW; 1.72 ± 0.13 kg) and one pig from each pair was randomly selected as control (CON) and the other received a second injection (+Fe). At weaning on d 22, each piglet was anesthetized, and samples of liver and duodenum were taken from the anesthetized piglets and placed in DNA/RNA Shield (Zymo, Irvine, CA) for preservation until mRNA extraction. RNA-Seq libraries were sequenced to a depth of at least 30 million read pairs (150 bp paired-end sequencing) per sample. Differential Gene Expression data were analyzed with GeneSpring (Agilent, Santa Clara, CA) with a fold-change (FC) of |1.2| P < 0.05 (Table 1). Pathway analysis was conducted with Ingenuity Pathway Analysis (IPA QIAGEN, Redwood City, CA) software with Z-score cutoff of P < 0.05. In the duodenum 435 genes were significantly changed with a FC ≥|1.2| P < 0.05. Most notably, Claudin 1 and Claudin 2 were inversely affected by the second dose of iron. Claudin 1 (CLDN1, FC = 4.48, P = 0.0423) is responsible for cell-to-cell adhesion in the epithelial cell sheets and serves as a barrier to prevent water and solutes from passing through the paracellular space. Claudin 2 (CLDN2, FC = -1.41, P = 0.0097) was down regulated because it is expressed in cation leaky epithelia. In the liver 362 genes were expressed with a FC ≥|1.2| P < 0.05. The gene most affected by a second dose of 200 mg Fe was HAMP (Hepcidin Antimicrobial Peptide) with an FC of 40.8. HAMP is a liver-produced hormone that is the main circulating regulator of Fe absorption and distribution across tissues. It also controls the major flows of Fe into plasma by promoting endocytosis and degradation of ferroportin (SLC4A1). This leads to the retention of Fe in Fe-exporting cells and decreased flow of Fe into plasma. Metabolic pathway changes in the duodenum and liver provide evidence for the improved feed conversion and growth rates in pigs given two iron injections pre-weaning. In the duodenum, there is a down regulation of gluconeogenesis (P < 0.05). Concurrently, there is a decrease in the production of urea in the liver (P < 0.05). These observations show that there is less need for gluconeogenesis, thus less urea production from deaminated amino acids. The genomic and pathway analyses provided empirical evidence linking gene expression with phenotypic observations of piglet health and growth improvements.