Immunochromatographic assay for detection (2-benzothiazolylthio) methyl thiocyanate in food packaging paper materials

The residue of fungicides in food packaging paper materials is one of the important factors leading to food safety problems, which poses a serious threat to public health. (2-benzothiazolylthio) methyl thiocyanate (TCMTB) is an important anti-mildew fungicide, which is widely used in wood and paper industry. In order to ensure the health of consumers, it is of great significance to establish an efficient and sensitive rapid detection method for TCMTB in food packaging materials. In this study, we prepared monoclonal antibody (mAb) against TCMTB with the IC 50 value of 7.60 ng/mL, and subsequently developed an immunochromatographic assay (ICA) strip based on the mAb for the rapid detection of TCMTB residues. The calculated detection limit of the ICA strip in food packaging paper was 3.20 ng/g, and the recovery rate was within an acceptable range, ranging from 97.7% to 102.7%. Therefore, the developed ICA is expected to realize the rapid screening of TCMTB residues in food packaging paper.