<i>S</i> timulus-modulated <i>a</i> pproach to <i>s</i> teady- <i>s</i> tate: A new paradigm for event-related fMRI

Functional MRI (fMRI) studies discard the initial volumes acquired during the approach of the magnetization to its steady-state value. Here, we leverage the higher temporal signal-to-noise ratio (tSNR) of these initial volumes to increase the sensitivity of event-related fMRI experiments. To do this, we introduce Acquisition Free Periods (AFPs) that allow for the full recovery of the magnetization, followed by task or baseline acquisition blocks (AB) of fMRI volumes. An appropriately placed stimulus in the AFP produces a Blood Oxygenation-Level-Dependent (BOLD) response that peaks during the initial high tSNR phase of the AB, yielding up to a ∼50% reduction in the number of trials needed to achieve a given statistical threshold relative to conventional fMRI. The silent AFP can be exploited for the presentation of auditory stimuli or uncontaminated electrophysiological recording and its variable duration allows aperiodic stimulus or response-locked signal averaging as well as gating to physiology or motion.