Neural stem cell–derived extracellular vesicles purified by monolith chromatography retain stimulatory effect in in vitro scratch assay

The results indicate that monolith chromatography is a viable purification method for EVs derived from cell culture that does not detract from the product's ability to stimulate fibroblasts, suggesting that product functionality is conserved. Further work is needed in developing suitable downstream processes and analytics to achieve clinically relevant purities for injectable biologics.