New Developments in Single Particle Cryo-EM Data Processing in CryoSPARC

Single particle cryo-EM (cryo-electron microscopy) allows high-resolution imaging of macromolecular complexes in close-to- native state, at near-atomic resolutions. As cryo-EM makes rapid progress, some of the key challenges that remain are in dealing with conformational heterogeneity, obtaining high resolution structures of small and transmembrane proteins, and automating 3D structure determination for rapid drug discovery projects. In this work, we describe new algorithmic, software and workflow developments in these areas within CryoSPARC, one of the most widely used software systems for single particle data processing. In addition, we present results of processing challenging targets including GPCR complexes and flexible proteins.