Abstract 1396: Additional flexibility and choice with Annexin V StarBrightTM Dyes

Abstract Annexin V staining combined with a viability dye is the gold standard to detect apoptotic cells and necrotic cells by flow cytometry due to its quick and reliable detection of externalized phosphatidylserine on cell membranes. Annexin V staining can be readily combined with immunophenotyping using fluorescently labeled antibodies to obtain cell specific apoptosis information. Unfortunately, when it comes to choice of fluorescent label on Annexin V there are limited options, with traditional fluorophores such as FITC, PE and APC being prevalent. This can cause issues with spillover and spreading when multiplexing, with bright fluorescent proteins such as GFP and with viability dyes that have excitation by multiple lasers, leading to suboptimal data and an inability to expand panel size. Here we present data using StarBright Dye Annexin V conjugates. Not only are the dyes bright so you get excellent separation of positive and negative populations, but they are available in a range of eight excitation and emission wavelengths. StarBright Dyes have been selected to have spectral properties that avoid excitation and emission of common viability dyes, such as 7-AAD and propidium iodide, fluorescent proteins like GFP, and are proven to be compatible in large immunophenotyping panels. When combining with immunophenotyping panels you still have the flexibility and choice to apply best practice, even with larger panels, improving the data obtained. Simplify compensation and avoid resolution issues caused by excessive spreading by using Annexin V conjugated to StarBright Dyes in your flow cytometry panels. Citation Format: Michael Blundell, Ryan Mendoza, Sharon Sanderson. Additional flexibility and choice with Annexin V StarBrightTM Dyes [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 1396.