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BACKGROUND: Musculoskeletal anatomy knowledge is an integral component in physiotherapy education and practise. However, the rate of retention of musculoskeletal anatomy is not satisfactory according to recent evidence. The results of several studies has shown a decrease in musculoskeletal anatomy retention, which highlights that there is a need to evaluate the retention rates. So, the aim of this study was to find out the retention of musculoskeletal anatomy among undergraduate students of physiotherapy. MATERIAL & METHODS:. This cross-sectional study was conducted on students of physiotherapy from second through fifth year at Physiotherapy Colleges of Peshawar. Data to determine retention level of musculoskeletal anatomy was collected from a sample of students selected through multistage sampling, our questionnaire consists of four parts which includes demographic information, Blooming anatomy tool, carpal bone test and Tarsal bone test. Permission was taken from principals of all included colleges and consent was obtained from the participants. Data was analyzed using SPSS version 22. RESULTS: The result of overall retention in our study sample illustrated that only 11 (5.6%) students scored 76% or greater and attained high retention score while majority of students 105 (53.6%) scored low and were categorized as having mild retention. Number of students that scored moderate retention was 80 (40.8%).More than half of the students 107 (54%) had mild retention of the carpal bones. Majority of the students 73 (37.2%) scored 76% or greater (High retention) on tarsal bone test. CONCLUSION: The results of our study concluded that majority of students in different physiotherapy institutes depicted mild retention of anatomy. Lowest scores that students got were in Blooms anatomy tool while greater percentage of students scored high on tarsal bone test as compared to carpal bones. Clinical students demonstrated better anatomy retention as compared to preclinical students. KEY WORDS: Anatomy, musculoskeletal anatomy, knowledge, retention