Abstract 1520 Investigating the Interaction Between Yeast tRNA Modification Proteins Trm7 and Trm734

In the anticodon loop of tRNAPhe, the modification of nucleotide G34 is fundamental for translation in Saccharomyces cerevisiae, humans, and other eukaryotes. In S. cerevisiae, the 2'-O-methylation of G34 is done by the Trm7:Trm734 complex. We study this complex because it is not fully understood why Trm7 needs Trm734 to function. The TRM7 human homolog is FTSJ1, and the TRM734 homolog is WDR6. Loss of function mutations in FTSJ1 that cause loss of Gm34 modification in patients result in non-syndromic x-linked intellectual disability.