Protein structure alignment significance is often exaggerated

Machine learning has generated millions of high-quality predicted protein structures, creating a need for computationally efficient structure search algorithms and robust estimates of statistical significance at this scale. We show that unrelated proteins have a universal tendency towards convergent evolution of secondary and tertiary motifs, causing an excess of high-scoring false positive alignments. We investigate popular structure search and alignment algorithms, finding that previous methods routinely overestimate significance by up to six orders of magnitude. To address these issues, and to accommodate recent innovations in search algorithm design, we describe a novel method for estimating statistical significance. We show that its <i>E</i> -values are accurate, scale successfully with database size, and are robust against the (generally unknown) diversity of folds in the database. We implement our approach in an online structure search service based on Reseek at https://reseek.online .