Repurposing proteomic nanoLC-MS platforms for untargeted metabolomics: evaluating DIA and polarity switching performance in human plasma

20250 citationsJournal Article

Authors

Frederico Garcia Pinto · Universidade Federal de Viçosa

Alexander D. Giddey · Mohammed Bin Rashid University of Medicine and Health Sciences

Nesrin Mohamed · Mohammed Bin Rashid University of Medicine and Health Sciences

Rauda S. B. Almarri · University of Sharjah

Munazza Murtaza · Mohammed Bin Rashid University of Medicine and Health Sciences

Nasna Nassir · Mohammed Bin Rashid University of Medicine and Health Sciences

Abstract

The polarity switching DIA approach for metabolomics reduced sample consumption and analysis time, but led to fewer detected features and annotations compared to separate polarity runs. These findings support the use of unified nanoLC-MS platforms for integrated multi-omics analysis.

Topics & Keywords

Metabolomics and Mass Spectrometry Studies Advanced Proteomics Techniques and Applications Mass Spectrometry Techniques and Applications

Publication Details

Published in: Expert Review of Proteomics

Volume 22, Issue 8, pp. 307-314

DOI: 10.1080/14789450.2025.2537210

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Repurposing proteomic nanoLC-MS platforms for untargeted metabolomics: evaluating DIA and polarity switching performance in human plasma

Authors

Abstract

Topics & Keywords

Publication Details