SUMO proteases SPF1/2 inhibit seed filling and fatty acid biosynthesis by deSUMOylating WRI1

Abstract Seed filling and fatty acid biosynthesis are critical processes for seed development and oil accumulation in plants, regulated by intricate post-translational mechanisms. Here, we elucidate the molecular mechanism by which SUMO proteases SPF1 and SPF2 regulate seed filling and fatty acid biosynthesis by modulating the stability of the WRI1 protein through deSUMOylation in Arabidopsis . SPF1 and SPF2 were found to negatively regulate seed filling and oil accumulation by promoting WRI1 degradation via deSUMOylation, which enhances its interaction with BPM proteins and subsequent ubiquitination. Our findings establish a dynamic interplay between deSUMOylation and ubiquitination in controlling WRI1 stability, unveiling a novel mechanism for seed filling. These findings establish SPF1/2 as crucial factor in maintaining WRI1 stability and seed filling, providing valuable genetic resources and a theoretical foundation for improving seed quality and oil content in agricultural production.