Biomimetic aligned bacterial cellulose dressing containing polydeoxyribonucleotide for promoting wound healing

Chronic or non-healing wounds present a significant challenge in daily life, leading to increased interest in the design of wound dressings with enhanced healing capabilities. In this study, a bacterial cellulose (BC) membrane with an aligned structure was developed to guide cell migration and collagen orientation, serving as the primary component of the wound dressing. Polydeoxyribonucleotide (PDRN), a nucleic acid agent known for its excellent tissue-regenerative effect, were loaded into the aligned BC membrane. The obtained composite material (PDRN@BC aligned membrane) demonstrated aligned structure, high hydrophilicity, favorable air permeability, good mechanical properties, and sustained release of PDRN. The as-prepared membrane promoted cell spreading, proliferation and migration of L929 fibroblast <i>in vitro</i>, as well as angiogenesis <i>in ovo</i>. Application of the PDRN@BC aligned membrane <i>in vivo</i> significantly improved and speeded up wound healing by accelerating cell proliferation and migration, reducing inflammation, and facilitating angiogenesis. This innovative composite dressing, combining topographic and bioactive cues, holds promise to facilitate the regeneration of damaged skin tissue.