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Background: Triple negative breast cancer (TNBC) is an invasive subtype of breast cancer associated with various risk factors. It has poor prognosis due to lack of targeted therapies. About 60-80% of patients carrying BRCA1 gene mutations have TNBC phenotype and the frequency of BRCA1 mutation is 10-15% in TNBC. In Bangladesh, very few studies are conducted regarding this occurrence and it needs further evaluation. Our research aim is to identify the mutation in exon7 of BRCA1 gene in Bangladeshi women with TNBC. Method: This cross-sectional descriptive study was carried out in the Department of Biochemistry, Dhaka Medical College, Dhaka in collaboration with Institution for Population and Precision Health, The University of Chicago, USA from January 2022 to December 2022. Total thirty-four (34) Bangladeshi female TNBC patients were selected. Two ml venous blood was collected from arm vein, kept in Ethylenediaminetetraacetic acid (EDTA) containing tubes, preserved at -20 degree C and sample was sent to the University of Chicago on Dry ice. DNA library was prepared by using Twist Library Preparation EF Kit 2.0. For mutation detection, Next-generation sequencing method was used. Result: The mean ± SD age of patients was 43.71±9.84 years and BMI was 23.95±3.38. About 52.9% of female had history of taking contraceptive methods and 70.6% patients were multiparous, remaining 14.7% were nulliparous and 14.7% were primiparous. 5.9% patients had positive family history and No mutation was found in exon 7 of BRCA1 gene. The frequency of mutation was 0.0%, as this study population was relatively small. Conclusions: This result suggests that Bangladeshi women suffering from TNBC have no mutation in exon 7 of BRCA1 gene. A large-scale investigation is needed to establish these findings.