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The aim of the study is to comprehensively evaluate the acute and subchronic toxicity of the wound-healing gel Maclozol to determine its safe doses for use in veterinary practice. The studies were conducted on laboratory animals at the vivarium of the KSCZV. The reaction of laboratory rats to the administration of the drug and potential negative effects were studied. Acute toxicity was assessed with a single administration of the drug at a dose of 6000 mg/kg of body weight. An important aspect of research was the study of changes in the physiological state of laboratory rats, clinical signs such as animal activity, gastrointestinal tract function, as well as skin and coat condition. For a deeper understanding of the long-term effects of the use of the gel Maclozol, a subchronic toxicity study was conducted. During the experiment, three different dosages of the drug (600; 300 and 120 mg/kg) were used, as well as a placebo for 30 days. The tests conducted to determine the acute and subtoxic effects of the gel Maclozol established that the drug is safe. Observations showed a slight deterioration in the rats' condition immediately after administration due to manipulations, but the animals' condition recovered within 30–60 minutes. The animals' body weight gain in the subchronic experiment ranged from 8.4 to 8.8 % in the experimental groups. Activity remained high throughout the experiment. The absence of signs of diarrhea and the normal state of the gastrointestinal tract confirmed the absence of toxic effects. The use of the gel did not lead to any significant changes in the hematological and biochemical parameters of the animals compared to the control group. Long-term use of this wound healing agent is safe and does not cause toxic effects on the animal body. This made it possible to conclude that the levels of use of the drug for 30 days are safe. The Maklozol gel was classified as low-toxic and assigned to the 4th category (GOST 12.1.007-76 "Harmful substances. Classification and general safety requirements").