Development of a non-invasive diagnostic method for pathogenic RNA viruses using sebum wiped from the cat’s body surface

Abstract The development of non-invasive diagnostic methods for zoonotic viral infections is increasingly important for both animal welfare and public health. Sebum-based diagnostic methods using commercial oil-blotting films have been applied to SARS-CoV-2 detection in humans, yet equivalent strategies for veterinary use remain largely unexplored. Severe fever with thrombocytopenia syndrome (SFTS), caused by the SFTS virus (SFTSV), presents a major health threat in Asia—especially in Japan, where multiple cases of cat-to-veterinarian transmission have been reported. To address the need for safer diagnostics, we sought to establish a sebum-based RNA virus detection method for cats. We designed primers that efficiently detected RNA from feline sebum while distinguishing it from human and feline DNA/RNA. Using this platform, we determined the ear to be the optimal sebum collection site and confirmed that feline immunodeficiency virus (FIV) RNA can be reliably identified from ear sebum with sensitivity comparable to conventional blood-based testing. In addition, we detected SFTSV RNA from sebum samples of infected cat. Our findings introduce a minimally invasive, safe diagnostic platform for feline viral infections, reducing animal distress while safeguarding veterinarians and pet owners from zoonotic risks. This strategy marks an important step toward realizing the One Health framework by advancing the well-being of both animals and humans.