Effect of Different Training Methods on SIRT1 Gene Expression in Subcutaneous Adipose Tissue of Male Wistar Rats

Objective: SIRT1 is a key regulator of adipose tissue's energy balance, inflammation, and mitochondrial function. This study was designed to investigate the effect of different training modalities, Moderate-Intensity Aerobic Training (MIAT), High-Intensity Aerobic Training (HIAT), and High-Intensity Interval Training (HIIT), on SIRT1 gene expression in subcutaneous adipose tissue of male Wistar rats. Materials and Methods: Thirty-two 8-week-old male Wistar rats (237 ± 33 g) were randomly assigned to four groups (n= 8): Control, MIAT, HIAT, and HIIT. Animals were housed under controlled conditions and had free access to food and water. Training protocols were performed five times per week for eight weeks. After 12 hours of fasting and 24 hours post-intervention, subcutaneous abdominal adipose tissue was collected. SIRT1 gene expression was analyzed using RT-PCR. Data were statistically evaluated using one-way ANOVA and LSD post hoc test (P< 0.05) in SPSS-22. Results: The results showed that all three training models - MIAT (P= 0.023), HIAT (P= 0.001), and HIIT (P= 0.003) - significantly increased SIRT1 gene expression compared to the control group. Conclusion: Although all training modalities significantly increased SIRT1 gene expression compared to the control group, the HIAT group showed the highest mean expression level. However, no statistically significant difference was observed between the training groups.