LbL-Antibody Embedded Gold Mesh: An Effective Method for Early Detection of Circulating Tumor Cells

This antibody network reduced the effective pore size, thereby improving both capture efficiency and specificity for CTCs. In a cell line separation study, meshes with a trilayered antibody network demonstrated a capture efficiency of 65% compared to 26% for those with a single layer. In tests using clinical samples, the trilayered antibody network achieved 100% accuracy, whereas the single-layer configuration only reached 40%. The multilayered antibody network shows strong potential for enhancing widely used immunosensors.