Cx43 Cell: DNA Validation

Recently, a proposal for a Cx43 was submitted by Ahmed Sihorwala from the Balardi Lab at UT-Austin based on previously published work Sihorwala et al. (2023). A Cx43 Module represents a pore-forming system that offers an alternative to α-hemolysin and provides a road towards developing tissue-like assemblies of synthetic cells that take advantage of Cx43’s ability to form hexameric connexons. In response, we have synthesized Nucleus-compatible plasmid constructs based on the sequences described in that work pOpen-pT7-Cx43 and pOpen-pT7-Cx43-eGFP that can be distributed under the Open MTA. This Developer Note presents validation data for those constructs, verifying successful membrane insertion and pore formation.