Molecular hydrogen in the technology of artificial insemination of cows

Background. Cryopreservation of sperm from cattle is an effective method of breeding work. To increase the efficiency of cryopreservation, the improvement of synthetic diluents for freezing sperm is required. Special attention is paid to the search for new cryoprotectors capable of increasing sperm survival and preserving their fertility during cryopreservation. Purpose. Evaluation of the effectiveness of artificial insemination of cows with sperm containing molecular hydrogen. Materials and methods. For the experiment, 2 groups of animals were selected, 50 heads each. The cows of the control group were inseminated with sperm frozen in a BioXcell medium, and the animals of the experimental group with sperm frozen in a BioXcell medium with molecular hydrogen. Insemination of cows was carried out by a group method by synchronizing sexual hunting according to the "Ovsinh" scheme. The effectiveness of insemination was evaluated on the basis of rectal ultrasound examination data after insemination. A comparison was made of the effectiveness of artificial insemination of cows with sperm cryopreserved in an improved medium containing molecular hydrogen with the indicators of fertilization obtained using sperm frozen in an environment without molecular hydrogen. Results. Fertilization of cows with sperm containing molecular hydrogen increased by 11.5% after 3 months compared with the use of cryopreserved sperm without molecular hydrogen. Conclusion. The results of the experiments indicate that the use of molecular hydrogen in the composition of the medium for diluting the seed makes it possible to increase the fertilizing ability of bull sperm and increase the fertilization rates of cows during artificial insemination.