Dose-dependent benefits of dietary yeast cell wall supplementation on the growth performance and stress resilience of the European seabass (Dicentrarchus labrax)

Preventive health management is central to modern aquaculture practices, within which functional feed additives are a key tool to optimising fish robustness and disease resilience. Despite being well documented, only few studies have assessed the dose-effect of graded levels of S. cerevisiae yeast cell wall (YCW) on fish performance and mucosal barrier immunity. A 9-week trial was conducted to assess the impact of graded levels of YCW on the performance and mucosal barriers of the European seabass under period: (1) non-challenge (43 days) then (2) with an applied husbandry stressors (20 days) in the form of high stocking density, low water oxygen level, and repetitive handling. The four experimental diets were produced by incorporating graded levels of YCW (0.0, control; 1.5; 3.0 or 4.5 kg/MT) which were top dressed on the basal diet prior extrusion. At the end of the period 1, fish supplemented with YCW at 4.5 kg/MT revealed a 5% increase in body-weight compared to the control. Likewise, throughout the experimental period SGR and FCR were also improved by YCW supplementation (+ 5.8% and + 8.3% with YCW4.5, respectively). Supplemented diets increased intraepithelial lymphocytes density and the highest level of intake was associated with increased skin mucus secretion (+ 31% with YCW 4.5 kg/MT). Finally, we observed a linear relationship between fecal calprotectin-like levels and the YCW gradient: a positive correlation during period 1 and a negative correlation during period 2. In contrast, YCW supplementation levels were characterized by a positive linear relationship with fecal mucins across both periods. These results provide novel insights on the benefits and associated mechanisms of YCW extracts and reveal an optimal dose response with inclusion levels between 3.0 and 4.5 kg/MT. This study demonstrates that YCW dietary inclusion enhances growth performance, feed utilization, and mucosal immune barrier function in European seabass, with improved stress resilience evidenced by biomarkers fecal calprotectin-like and fecal mucins.