Nanoencapsulated gamma-oryzanol enhances fibroblast proliferation, migration, and reduces oxidative stress in H2O2-induced cellular aging model

This study investigates the impact of NanoEncapsulated Gamma Oryzanol (NEGO) on H 2 O 2 -induced NIH/3T3 fibroblasts and the enzymes associated with aging. NEGO was prepared using high-speed emulsification and characterized by UV–visible spectroscopy, dynamic light scattering (DLS), zeta potential, scanning and transmission electron microscopy techniques (SEM and TEM). Its antioxidant activity and enzyme inhibition (elastase, collagenase, hyaluronidase, and tyrosinase) were assessed, and in-silico docking was used to determine the binding propensity of Gamma Oryzanol (GO) with the enzymes. NEGO showed a remarkable absorption peak of GO at 326 nm, average size distribution of 162 nm (PDI: 0.2), large zeta potential (−51 mV), smooth, and spherical morphology. It showed a strong antioxidant effect and a dose-dependent inhibition of enzymes, and molecular docking confirmed the high binding affinity of GO (−7.0 to −8.1 kcal/mol) with the enzymes. NEGO enhanced cell viability, lowered reactive oxygen Species (ROS), and accelerated fibroblast migration. These findings highlight NEGO's promise as a natural, food-derived approach to mitigate ROS-induced cellular damage. • Formulated a nanoemulsion (NEGO) that markedly enhances GO solubility and stability. • NEGO exhibited superior antioxidant activity by lowering intracellular ROS. • NEGO showed potent inhibition of collagenase, elastase, hyaluronidase, and tyrosinase. • NEGO enhanced fibroblast proliferation, migration, and wound healing.