Method Validation of Pesticides by Gas Chromatograph

The organochlorine Pesticides are more potent due to their persistence and stability and pesticides like Aldrin, Endosulfan and Heptachlor have been classified as persistent organic pollutant (POP), with restrictive use. The pesticides are banned and not in use. Central Agmark Laboratory, Nagpur 10 rice flour samples collected from all over to detect the presence of these pesticides in GC. Method validation of these pesticides was done. The LOD for the validated method was 0.00030, 0.00013 and 0.00015mg/ml respectively for Endosulfan, Aldrin and Heptachlor. The LOQ as per S/N ratio was 0.000055mg/ml, 0.000028mg/ml, 0.0000267 mg/ml respectively for Endosulfan, Aldrin and Heptachlor. Endosulfan was not detected in any of the sample, Aldrin was detected in one sample as 0.004 ppm. Heptachlor was detected in all the analysed samples and 60% of the samples were more than 0.01 ppm. Keywords: Endosulfan, Aldrin, Heptachlor, Rice flour,