Proteomic and metabolomic profiling reveals the fungicidal mechanisms of Microsporum canis in response to methylene blue-mediated photodynamic therapy

The results indicate that the efficacy of MB-PDT stems from a multi-target mechanism that simultaneously damages cellular structures, attenuates virulence, and dismantles the fungus's metabolic and translational capacity. This contrasts sharply with single-target conventional antifungals, providing a strong molecular rationale for its low potential to induce resistance. This study offers a comprehensive molecular blueprint for the action of MB-PDT against <i>M. canis</i>, strongly supporting its development as a durable therapeutic strategy for dermatophytosis.