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Experimental data were generated between 2021–2025 under controlled laboratory conditions. Methodology: Bacterial Isolation and Identification Bacterial strains were isolated from the rhizosphere of Phragmites australis collected in 2021 from a phenol-contaminated pond (Staw Kalina, southern Poland). Root suspensions were enriched in basal salt medium (BSM) supplemented with bisphenols (BPA, BPS, BPF; 1–10 mg L⁻¹). Morphologically distinct strains were purified and screened for plant growth-promoting (PGP) traits, including phosphate solubilization, siderophore and IAA production, ACC deaminase activity, cellulolytic activity, motility, and biosurfactant production. Two strains (BP1 and BP2) were selected for further experiments. Identification was performed using whole-cell fatty acid profiling and multilocus sequence analysis (16S rRNA, gyrB, rpoD), followed by ANI comparison with type strains. Floating Treatment Wetland (FTW) Experiment FTW microcosms (20 L tanks) were constructed using P. australis and pond water artificially contaminated with BPA and BPS (5 mg L⁻¹ each) and BPF (2.5 mg L⁻¹). Five treatments were established: plant control, natural attenuation, bioaugmentation, phytoremediation, and bacterial-assisted phytoremediation. Microcosms were maintained at 22°C (16/8 h light/dark cycle) for 21 days with three biological replicates per treatment. Bacterial strains were immobilized in alginate beads prior to inoculation. Bisphenol Quantification and Removal Analysis Bisphenol concentrations were determined using HPLC-UV following solid-phase extraction (SPE). Removal efficiency (RE%) and biodegradation percentage (Bp%) were calculated considering adsorption, bioaccumulation, and abiotic removal. Kinetic modeling followed a pseudo-first-order model. Computational analyses were performed in Python (pandas, NumPy). Plant and Water Analyses Water quality parameters (pH, DO, TDS, TSS, COD, TOC, BOD₇) were measured using standardized analytical procedures. Plant physiological status was assessed through biomass determination, oxidative stress markers (H₂O₂, MDA, catalase activity), chlorophyll fluorescence, and RT-qPCR analysis of photosynthesis-related genes. Phytotoxicity of treated water was evaluated using Pisum sativum germination assays. Statistical Analysis Data were analyzed using two-way ANOVA/MANOVA with post hoc tests (p < 0.05). Data are provided in Excel (.xlsx), PDF, PNG, and IPBYN formats. The dataset includes: Plant growth-promoting (PGP) bacterial traits: – Indole-3-acetic acid (IAA) production – ACC deaminase activity – Phosphate solubilization – Biosurfactant production Water quality parameters (COD, BOD, pH, DO, TSS, VDS, TOC) Plant biometric parameters (fresh weight, root length, hypocotyl length) Toxicity bioassay data (seed germination and growth measurements) Gene expression data (qPCR results including Ct, ΔCt, ΔΔCt, and relative expression 2^-ΔΔCt) Chlorophyll fluorescence parameters (OJIP test measurements) Kinetic modelling The dataset contains raw and processed experimental data generated during the study. File Structure: Each Excel file corresponds to a specific experimental component or figure. Files contain labeled worksheets. Most of variables and measurement units are described here: Polish name English name Description Unit Szczep Strain Bacterial strain identifier - Układ Treatment Experimental treatment group - Powtórzenie Replicate Biological replicate number - Korzeń_długość_cm Root_length_cm Root length measured in centimeters cm Hypokotyl_długość_cm Hypocotyl_length_cm Hypocotyl length measured in centimeters cm Mokra masa_g Fresh_weight_g Fresh plant biomass in grams g Sucha masa_g Dry_weight_g Dry plant biomass in grams g IAA_koncentracja_ug_mL IAA_concentration_ug_mL Indole-3-acetic acid concentration (µg mL⁻¹) µg mL⁻¹ ACC_koncentracja_umol_mL ACC_concentration_umol_mL ACC concentration (µmol mL⁻¹) µmol mL⁻¹ Przejaśnienie Halo_zone_mm Halo zone diameter indicating phosphate solubilization (mm) mm Kolonia Colony_diameter_cm Bacterial colony diameter (cm) cm Absorbancja Absorbance_λ Spectrophotometric absorbance at specified wavelength unitless Ct Ct_value Cycle threshold value in qPCR cycles ΔCt ΔCt Delta Ct (Ct_target − Ct_reference) cycles ΔΔCt ΔΔCt Delta Delta Ct (ΔCt_sample − ΔCt_control) cycles Relatywna ekspresja_2^-ΔΔCt Relative_expression_2^-ΔΔCt Relative gene expression calculated using 2^-ΔΔCt method fold change