Supporting dataset (Figures 1–5, Table 1): External pH effects on bovine sperm motility and mitochondrial function

This record provides supporting materials for the manuscript: “The importance of external pH level in sperm journey: A new perspective on sperm motility and mitochondrial function: a bovine model study”(intended submission: Human Fertility). Contents- Figures 1–5 (TIFF): motility and kinematic changes across extracellular pH (CASA) and mitochondrial membrane potential (MMP; TMRE-based fluorescence).- Table 1 (XLSX): effects of extracellular pH on sperm motility and kinematics (mean±SD; n=30 frozen semen samples from one bull; 60 technical replicates per condition). Group differences were tested using one-way ANOVA (or Kruskal–Wallis if assumptions were violated). Multiple comparisons were controlled by Tukey’s HSD or Dunn’s test with BH adjustment (q<0.05).- README.txt: file list and brief notes. Experimental summaryCryopreserved bovine semen was assessed under extracellular pH 4.0–8.0. Motility/kinematic parameters (CASA) and mitochondrial membrane potential (TMRE-based fluorescence) showed pH-dependent changes. Embargo / accessThis record is deposited for peer review and manuscript evaluation. Access is restricted under embargo until publication (or until the embargo is lifted). Public release will follow the repository embargo settings and the manuscript’s data availability statement. Keywords: sperm motility; CASA; mitochondrial membrane potential; TMRE; extracellular pH; bovine; cryopreserved semen; sperm kinematics; VAP; VSL; VCL