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Gravity-driven column chromatography is a widespread technique used across many disciplines, including chemistry, biosciences, geosciences, engineering, industry, and medicine. The purpose is to separate out specific (suites of) compounds from a mixed solution. A lack of commercially available or suitable racks means that many users resort to making their own custom racks for their research needs. Often, these can be inefficient in their use of valuable bench or fume-hood space, limiting the number of columns that can be run simultaneously and thus significantly reducing laboratory throughput over time. Furthermore, many do not have a specialised vial holder, and misalignments between the vials and the end of the column can result in lost extracts, and increased error in any subsequent data. Here we present an open-source design for a gravity-driven column chromatography rack and vial holders for which the component parts can be entirely laser-cut, or a combination of laser-cut and 3D printed, from materials of the user’s choice. These components can be assembled without the need for specialised tools and can be flat-packed for easy shipping and transport. The vial holders are constrained laterally, and embedded alignment features facilitate precise positioning of the column above the collection vial. This low-cost design is intended as a complementary alternative to existing robust metal systems and aims to improve efficiency and reduce sample loss for users of gravity-driven column chromatography. All components are designed to assemble without fasteners or specialised tools, and laser-cut elements can be flat-packed for storage or transport. The rack supports up to ten gravity-driven chromatography columns with apertures and overall rack height sized for standard short (~150mm) glass Pasteur pipettes. Integrated vial holders accommodate four standard 4 mL glass vials per column, allowing the storage of one total lipid extract and the collection of three subsequent fractions. Each vial holder is laterally constrained within base tracks and individually numbered to correspond with the numbered column positions above, reducing the risk of misalignment. Alignment features are present along the top surface of the laser-cut version, and these lines can be extended manually, engraving into the vertical sides of the laser-cut version (this can be simply scratched in with a metal tool such as a nail). In the 3D printed option, these side alignment aids are integrated as cut-outs, enabling precise positioning beneath the column outlet, and eliminating the need for any post-fabrication modifications. The column rack is fabricated entirely by laser cutting. Acrylic and MDF were used in the column rack presented here due to their low cost and widespread availability, though users may substitute alternative materials, including metals, depending on solvent compatibility and contamination concerns. The vial holders may be produced using either laser-cut components or a 3D-printed design. For the 3D-printed option, either nylon or glass fibre reinforced nylon filaments have been used. These materials are more resistant to the organic solvents commonly utilised in gravity-driven column chromatography than alternative filament materials such as PLA, ABS or resin. Slots incorporated into the upper plate allow tweezers or similar tools to be stored upright during column preparation, keeping frequently used tools clean and accessible. Furthermore, a removable base infill provides a flat surface for cleaning steps and can be removed during fraction collection. Vial slots embedded within the base plate facilitate the safer storage of additional vials. Based on production in Denmark using institutional maker spaces, costs are estimated as: • Approximate total cost for fully laser-cut rack and vial holders - €23• Approximate total cost for laser-cut rack and 3D printed nylon vial holders - €75 However, material cost, institutional fees and machine availability will dictate the real costs associated with producing this equipment. Below are further details relating to the files included in this repository: For simple manufacture (DXF and STL files, ready to use) 10 x files for 3D-printed vial holders, labelled 1-10 inclusive (Vaile holder 1 .STL, Vaile holder 2 .STL, etc.) 1 x column chromatography rack and laser-cut vial holders (gravity driven chromatography column rack.DXF) Laser-cut column chromatography rack - files for modification (STEP files) 1 x file for assembly view of all components of the laser-cut column chromatography rack (Column chromatography rack - ASSEMBLY.STEP) 1 x file for column chromatography rack main base plate, with attachment points for the legs (Rack_main base-plate_bottom female.STEP) 1x file for column chromatography rack infill/removable section of the base-plate (Rack_base-plate infill_bottom male.STEP) 1 x file for the supports/legs of the column chromatography rack, four of which are needed per rack (Rack_leg (cut four for one rack).STEP) 1 x file for the middle shelf of the column chromatography rack, which supports the lower portions of the columns (Rack_middle shelf.STEP) 1 x file for the top shelf of the column chromatography rack (Rack_top shelf.STEP) 3-D printed vial holder - file for modification (STEP file) 1 x file for the 3D printed version of the vial holder (Vial holder for 3D print.STEP) Laser-cut vial holder - files for modification (STEP files) 1 x file for assembly view of all components for the laser-cut vial holder (Vial holder for laser cut - ASSEMBLY.STEP) 1 x file for the bottom four components of the laser-cut vial holder (Vial holder_bottom (cut four for one holder).STEP) 1 x file for the top two components of the laser-cut vial holder (Vial holder_top (cut two for one holder).STEP) 1 x file for the peg component of the laser-cut vial holder, which holds the other layers together (Vial holder_locking peg (cut two for one holder).STEP) Supplementary files 1 x individual un-numbered vial holder file (One vial holder.DXF) 1 x AI file of column chromatography rack (Chromatography Column Rack.AI) 1 x assembly video for the column chromatography rack (Column chromatography stand.mp4) 1 x assembly video for the laser-cut version of the vial holder (One laser cut column holder.mp4) 20 x photographs demonstrating the completed look of the vials and rack holder (e.g. IMG_20260107_142916.jpg etc.) This gravity-driven column chromatography rack and vial holders were designed at The Geological Survey of Denmark and Greenland (GEUS) by C.A.W-T and E.V. during a post-doctoral position, and an internship associated with an Erhvervsakademi København Product Development and Integrative Technology (BA) program, respectively. We thank Professor Camilla S. Andresen (Department of Glaciology & Climate, GEUS) for supporting this work in her capacity as PI and DFF1 grant holder for the post-doctoral research project, Gulfstream variability in a globally warming world – the forgotten Danish archive (10.46540/3103-00193B).