Biophysical Alterations of the Stratum Corneum Induced by Optical Clearing Agents: Implications for Efficiency and Safety Optimization

The findings of this study contribute to the identification of optimal OCA combinations - namely, a 25%-25%-50% water-ethanol-fructose solution and a mixture of 90% Omnipaque® with 10% DMSO, each applied for 5 min - for potential in vivo applications. Moreover, the results provide a strong foundation for future multimodal investigations aimed at developing advanced methods for visualizing biological tissues and organs and analyzing their biophysical parameters, in conjunction with controlled OC.