Mineral-Based Advanced Oxidation Processes for Enhancing the Removal of Antibiotic Resistance Genes from Domestic Wastewater

© 2025 The Authors. Published by American Chemical Society. Wastewater treatment plants (WWTPs) release antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) into the environment. Advanced oxidation processes (AOPs) can remove ARB and ARGs, but they often require impractically high chemical or energy use. Here, we explore a low-energy AOP that uses Fe-bearing clay mineral (NAu-1) either combined with H2O2 (H2O2/NAu-1) or as prereduced structural Fe (rNAu-1) to degrade selected ARGs (i.e., tetM, tetQ, and blaOXA-10), int1 (a mobile genetic element), and the 16S rRNA gene in postsecondary WWTP effluents. Addition of H2O2/NAu-1 significantly increased tetM and int1 removals relative to UV irradiation and H2O2/UV (p ≤ 0.02). Removals increased with greater H2O2 doses and contact times, reaching maximum values of 1.2 and 2.3 log units at H2O2 doses of 0.26 and 10 mM and contact times of 4 and 8 h, respectively. Bacterial regrowth after 24 h of contact was probably due to H2O2 depletion. However, the addition of rNAu-1 achieved the highest removals, up to 2.9 log units after 0.5 h, and suppressed bacterial regrowth over 24 h. Similar removals were observed with rNAu-1 under oxic and anoxic conditions. Results show that mineral-based AOPs offer the potential for elevated ARG removal and lower chemical and energy demands in tertiary wastewater treatment.