The impact of serum estradiol on mature oocytes and pregnancy outcomes in IVF: retrospective observational study

Abstract Objective The study aims to assess the correlation of serum estradiol levels with the number of mature follicles, oocytes retrieved and mature oocytes and pregnancy rates in ART. Materials and methods An observational retrospective study was conducted in 2 years (2023–2024) in Ridge IVF centre, New Delhi, India. 200 women of age 23–40 years undergoing IVF/ICSI were included. Correlation of Serum Estradiol on the day of trigger to the number of mature follicles (size ≥ 14 mm), the number of oocytes retrieved, and the number of mature oocytes, Serum AMH, Estradiol per oocyte and Estradiol per mature oocyte on the day of trigger were assessed. The correlation of FORT and FOI with Serum AMH levels and Serum Estradiol levels were also assessed. Results The Serum Estradiol levels was positively correlated with mature follicles, oocytes retrieved and mature oocytes. Estradiol-to-oocyte and estradiol-to-mature oocyte ratios both increase with rising estradiol levels. 105 achieved a positive pregnancy, while 95 did not. The mean Serum E2 in the positive pregnancy group was 3444.4 ± 2780.35 pg/mL, compared to 3166.4 ± 2571.35 pg/mL in the negative pregnancy group. Conclusion Serum estradiol levels can be used as marker to assess the ovarian response of the patient and help in clinical decision-making and treatment strategies in reproductive medicine.