Additional file 3 of Efficacy of injectable versus topical formulation of ivermectin against Anopheles stephensi mosquitoes feeding on different body locations of treated Holstein calves

Supplementary Material 3.Figure S1. Experimental feeding of Anopheles stephensi mosquitoes on calves. A Laboratory-reared mosquitoes were aspirated into sturdy, screen-top cassettes modified from food storage containers (@ 120 per cage) and transported by automobile to the dairy facility. B The back, belly and hind leg of calves were shaved and the mosquito cassettes were strapped into place using plastic food wrap. C Mosquito feedings were conducted on back, belly and leg for multiple animals stanchioned in place. D Mosquitoes were allowed to feed for 15 to 30 min, then the plastic wrap was cut with scissors, cassettes carefully removed, and transported back to the laboratory for processing.