Quantitative results corresponding to the work "Genipin-crosslinked decellularized collagen tissue-like membranes. A biocompatibility and functional evaluation for tissue engineering"

This dataset corresponds to the quantitative data generated in the work entitled "Genipin-crosslinked decellularized collagen tissue-like membranes. A biocompatibility and functional evaluation for tissue engineering" AbstractDecellularized collagen matrices (DCM) are promising biomaterials for tissue regeneration due to their biocompatibility and structural functionality. This study evaluated the histomechanical performance, biocompatibility, and in vivo behavior of Genipin (GP)-crosslinked DCM to determine their suitability for tissue engineering applications. Porcine pericardium–derived DCM were treated with increasing GP concentrations (0.01%, 0.1%, 1%). Structural characterization, histomechanical testing, cell viability assays, and macrophage phenotyping were conducted. Based on mechanical performance, the 0.01% GP condition was selected for in vivo evaluation following subcutaneous implantation in BALB/cJRj mice. Local biocompatibility, systemic toxicity, and biodegradation at 10 and 30 days were assessed by histological and biochemical analyses, with untreated DCM as controls.GP treatment preserved the native 3D collagen architecture and significantly improved Young’s modulus, strain at fracture, and deformation, with the most favorable effect observed at 0.01%. All scaffolds were non‑cytotoxic and induced a macrophage shift toward a pro‑regenerative phenotype. In vivo, 0.01% GP‑crosslinked DCM exhibited enhanced biointegration, reduced biodegradation, and promoted an M2‑type response, without systemic alterations in blood chemistry or organ histology. These findings demonstrate that low‑dose GP crosslinking effectively enhances the mechanical stability of DCM while maintaining excellent biointegration and safety. This approach shows strong potential for the development of collagen‑based scaffolds for applications in skin, oral mucosa, palate, urethra, and corneal tissue engineering.