Optimization of BSA Column Gradient Concentrations on Post-Thaw Quality of Sexed Spermatozoa in Bali Bulls

This study evaluated post-thaw semen quality and the proportions of X- and Y bearing spermatozoa in Bali cattle semen sexed by albumin of bovine serum albumin (BSA) column using two different gradients. In this study, frozen semen from a Bali bull was used, with three treatments: T0 (non-sexed frozen semen as a control), T1 (BSA 5%:8%), and T2 (BSA 5%:10%). The parameters observed were individual motility, viability, abnormality, concentration, total motile sperm (TSM), and sperm proportion. The results showed that sperm motility in the upper fraction (UF) was highest under T2 (41.57%), whereas in the lower fraction (LF) it peaked under T1 (24.15%). Sperm viability was highest in the UF under T1 (57.69%) and in the LF under T1 (39.02%). The lowest abnormality was observed in the UF under T2 (4.64%) and in the LF under T1 (7.27%). Sperm concentration reached 30.14 million/straw in the UF under T2 and 22.27 million/straw in the LF under T2. Total sperm motility (TSM) was highest in the UF under T2 (12.57 million/straw) and in the LF under P1 (4.76 million/straw). The sperm proportions of the UF reached 75% X (T1) and the LF 81.71% Y (T1). Overall, the upper fraction obtained from the 5%:8% BSA gradient provided acceptable post-thaw sperm quality and adequate X/Y sperm enrichment, supporting its suitability for artificial insemination.