Next Generation Sequencing of Tracheal Aspirates in Children With Tracheostomy A Prospective Case‐Control Study

NGS can effectively identify a wide spectrum of DNA-based pathogens (bacteria, viruses, and fungi) in the tracheal aspirates of children. Tracheostomized children were significantly more likely to be colonized by difficult-to-treat bacteria. Future research is required to evaluate the clinical benefits of NGS compared with standard microbiological procedures.