Identification of public neoantigens with broad HLA class I coverage as candidates for off-the-shelf cancer vaccine development in colorectal cancer

These findings identify TP53_R273H as a clinically relevant shared neoantigen in CRC and support the translational feasibility of a population-tailored OTS neoantigen panel. Our integrative computational and experimental framework highlights the importance of combining population-level HLA coverage with functional validation to advance broadly applicable neoantigen-based immunotherapies.