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Gridded meteorological data sources, such as reanalysis datasets, are increasingly used to estimate evapotranspiration, a key variable for surface water-budget analyses at regional and national scales and for assessing plant water requirements for irrigation. This study, conducted over mainland Portugal for the 44-year reference period from 1980 to 2023, first presents a comprehensive comparative analysis of the spatial patterns of potential (Ep) and reference (Eto) evapotranspiration at a 0.1° spatial resolution using daily data. Estimates derived from two high-resolution datasets (GLEAM and ERA5-Land) are compared with those obtained from the Thornthwaite, Hargreaves–Samani, and Penman–Monteith models. Secondly, trend analyses of Eto magnitudes on a monthly and annual basis in a gridded format were conducted. The resulting spatial distributions of Ep and Eto show higher values in milder and flatter southern Portugal and lower values in the cooler and more mountainous northern regions, in agreement with existing knowledge. The Penman–Monteith model exhibited the highest reliability, while the Thornthwaite model generally underestimated evapotranspiration across the country, and the Hargreaves–Samani model showed underestimation in coastal areas. Trend analysis of Eto indicates an overall increase in atmospheric evaporative demand over the full study period, with a more pronounced rise during the recent 22-year period (2002–2023) compared with the earlier period (1980–2001). These increases are statistically significant in August and October and may reflect a climate shift towards a progressively longer dry season. Understanding how changes in evapotranspiration affect hydrological processes—including surface water availability, river discharge, reservoir performance, and crop requirement—is critical. This study aims to contribute to addressing these emerging challenges.