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Accurate spatial information on individual tree locations is essential for precision forestry, the integration of field and remote sensing data, and tree-level forest analyses. This study compared the positional accuracy and tree identification performance of four tree-mapping approaches: legacy paper maps, a pseudolite-based field positioning system (TerraHärp), drone-based laser scanning, and mobile laser scanning (MLS). The analysis was conducted in five long-term experimental forest sites in Lithuania, comprising pine- and spruce-dominated stands with varying stand densities. Tree locations derived from legacy maps and the TerraHärp system were compared to assess systematic and random positional discrepancies. TerraHärp-derived tree positions were subsequently used as a reference to evaluate the laser scanning-based methods. Positional accuracy was assessed using Hotelling’s T2 test, root-mean-square error, and the National Standard for Spatial Data Accuracy (NSSDA), while spatial autocorrelation of deviations was examined using Moran’s I. The results indicated that discrepancies between TerraHärp and legacy maps were dominated by systematic horizontal shifts in the historical maps, whereas random positional variability was relatively small and consistent across stand types. Drone-based laser scanning showed a strong dependence of tree identification accuracy on stand density and mean tree diameter. Overall, CHM-based segmentation yielded more accurate tree identification than 3D point cloud segmentation, with mean F1-scores of 0.78 and 0.72, respectively. Positional accuracy varied by method, with the largest errors from CHM apexes and highest 3D point cloud points (mean NSSDA ≈ 1.8–2.0 m), improved accuracy using the lowest 3D cluster points (1.45–1.72 m), and the highest accuracy achieved using mobile laser scanning (mean NSSDA 0.76–0.90 m; >95% of trees within 1 m). These results demonstrate that pseudolite-based field mapping provides a reliable reference for high-precision tree location and for integrating field and laser scanning data in managed conifer stands.