Anti-Inflammatory Effect of <i>Aniba rosaeodora</i> Essential Oil-Loaded Chitosan Membrane

Bioactive chitosan (CH) membranes containing 0.5, 2.5, and 5.0% w/w of <i>Aniba rosaeodora</i> essential oil (ArEO), rich in linalool (82.9%) and cis-linalool oxide (2.9%) were developed using the casting method. Fourier transform infrared spectroscopy (FTIR) results demonstrated that CH chains interacted with ArEO, leading to increased thermal stability and preventing isolated ArEO degradation. ArEO concentrations did not influence membrane morphology. Incorporating ArEO into the formulated membranes did not significantly alter their Young's modulus (41.84-48.00 MPa), which remains within the skin's modulus of elasticity range (0.02 to 57 MPa). They also exhibited mucoadhesion for tissue adhesion, with no significant difference with adding ArEO (0.135-0.161 N). Additionally, ArEO incorporated into the developed wound dressings significantly reduced nitrite levels in the in vitro inflammation model. Tumor necrosis factor-alpha (TNF-α) levels in lipopolysaccharide (LPS)-stimulated macrophages were significantly reduced at all ArEO concentrations in the wound dressings. The developed membranes may represent a promising alternative for wound dressings with anti-inflammatory properties.