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This article explores quesito de hoja, a product commercialized in the department of Antioquia, Colombia, as part of the region’s and the country’s gastronomic heritage. It is seen as a cultural expression that resists dairy industrialization in the municipalities of San Pedro de los Milagros and La Unión, preserving food security through a traditional product. For this project, a qualitative and ethnographic methodology was implemented, including participant observation, semi-structured interviews with producers, and photographic documentation, with the aim of strengthening the intangible culture associated with the artisanal production of this dairy derivative. This study presents practical evidence passed down through generations, connections woven into the territory, and gustatory memories now at risk of extinction due to sanitary regulations, agro-industrial production models seeking standardization, and a general lack of interest in safeguarding and sharing ancestral culinary knowledge. Consequently, the research addresses the conflict between tradition and modernity within the production process, as well as the challenges involved in safeguarding quesito de hoja in both rural and urban contexts. Finally, the article outlines strategies aimed at protecting and marketing this product, while also promoting its cultural and economic recognition, proposing initiatives for sustainable tourism experiences centered around territories rich in tradition and culinary craftsmanship in the production of Antioquian quesito.