Campylobacter jejuni culture method v1

Campylobacter jejuni is an important cause of foodborne illness in humans and animals. Rapid detection of animals shedding C. jejuni is a key step in limiting the scope of outbreaks and reducing the potential exposure of humans and other animals. Culture methods are needed for the recovery of isolates for antimicrobial susceptibility testing and whole genome sequencing efforts to monitor for antimicrobial resistance. This method was developed to provide a standard method for the culture of C. jejuni from feces from veterinary patients. This culture method is based on using Campy-Cefex and modified charcoal cefoperazone deoxycholate agar supplemented with 8 mg/L cefoperazone, 4 mg/L amphotericin B, and 10 mg/L teicoplanin (mCCDA-CAT) for selection of C. jejuni. Validation data (in-house and by an independent laboratory via collaborative study such as Randomized Blinded Method Test) are available upon request.