Korrelative Mikroskopie: Funktion und Struktur in einer Methode vereint

Abstract Many cellular processes are observed by light microscopy, whereas ultrastructure requires electron microscopy (EM). Correlative light and electron microscopy (CLEM) combines functional fluorescence signals with EM ultrastructural resolution in the same sample, enabling precise correlation. We present an optimized CLEM workflow for plant samples combining high-pressure freezing, freeze substitution, confocal and EM to analyze the function and structure of dynamic endomembrane compartments.