Spatial Profiling of Germinal Centers in Mouse Secondary Lymphoid Organs Using MACSima Imaging Cyclic Staining Technology

We developed a 50-plex imaging protocol using the MACSima platform to characterize the microarchitecture of germinal centers in secondary lymphoid tissues. This workflow combines tissue processing, automated cyclic imaging, image preprocessing, and a new analysis method that detects morphological changes in irregularly shaped stromal cells.