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Objectives. This study aimed to evaluate the psychometric properties of the EPOCH Measure of Adolescent Well-Being in a large sample of Czech high school students.Sample and settings. Data were collected from 2,967 adolescents (15 to 19 years old) as part of a broader school-based survey conducted across 13 secondary schools in the Czech Republic.Hypotheses. The authors hypothesized that the Czech version of EPOCH would replicate the original five-factor structure and demonstrate good internal consistency and construct validity.Analyses. Analyses included item-level descriptives, test–retest reliability, Mokken scaling, confirmatory factor analysis (CFA), measurement invariance across gender and age groups, and correlations with related constructs.Results. The results confirmed the five-factor model with good fit indices and demonstrated adequate internal consistency (McDonald’s ω ranging from .75 to .84). Multi-group CFA supported configural, metric, and scalar invariance, with partial support for strict invariance. EPOCH subscales correlated meaningfully with external variables, in line with theoretical expectations, supporting the instrument’s nomological validity.Limitations. The cross-sectional design represents a limitation, restricting conclusions about temporal stability and predictive validity. While the findings provide strong evidence that the Czech version of the EPOCH is a reliable and valid measure of adolescent well-being suitable for use in school-based research and practice, further studies are recommended to reinforce its nomological validity. Given the current selection of external validation measures – such as self-esteem, depressive symptoms, and satisfaction of basic psychological needs in physical education – future research should expand the scope of external variables to more comprehensively examine the theoretical network surrounding adolescent well-being.