PT1:01 IFN-alpha-stimulated monocyte secretion of B cell activating factor (BAFF) is mediated through autophagy and extracellular vesicles in systemic lupus erythematosus (SLE)

PT1:01 Figure 1 Autophagy -dependent secretion of BAFF in SLE patient-derived or IFNa stimulated CD14+ monocytes.(a) Representative confocal image of immunofluorescence microscopy of anti-LC3A/B, anti-CD63 and anti-BAFF (green: BAFF, red: LC3A/B, magenta: CD63) blue: 4',6-diamidino-2-phenylindole (DAPI)/DNA) are shown.Scale bars: 10m.(a,b) Pearson's co-localization coefficient for BAFF CD63 and LC3A/B.Each dot represents an independent SLE donor from 100 cells each.(n=7) and bar plots show the mean SEM expression.**** P < 0.0001 (two-tailed, Mann-Whitney test).(c) BAFF secreted levels by CD14+ monocytes cultured with 20% SEL active sera +/-Bafilomycin or stimulated with IFNa (c).