Isolation and characterization of Japanese encephalitis virus genotype I from pig provides evidence for the presence of the virus in nasal secretion and co-circulation of JEV genotypes in Assam, India

The findings underscore that JEV GI is circulating in Assam and there is need for strengthened JEV surveillance in swine to monitor genotype shifts, understand viral evolution, and generate field isolates critical for vaccine evaluation and preparedness against emerging JEV genotypes. The study also demonstrates the feasibility of using nasal swabs for virus detection and isolation thereby providing evidence for the presence of JEV in nasal secretion of naturally infected pigs.