HPLC-MS kaemferol and its glycosides identification

The article presents the results of kaempferol and its glycosides identification in plant extracts using the method of high-performance chromatography-mass spectrometry (HPLC-MS). The study is focused on the selection and optimization of chromatographic conditions for efficient separation and identification of these flavonoids with high biological activity, as well as at searching for new types of plant raw materials for the pharmaceutical industry of the Republic of Belarus. The use of the mass-spectrometric and spectrophotometric detection ensured high sensitivity and selectivity in the identification of kaempferol and its glycosides. Astragalin in an amount of more than 2.20 mg/g of absolutely dry raw material is contained in the extracts of Asclepias incarnate L., Geranium pratense L. and Glycyrrhiza uralensis Fisch. ex DC. Three glycosides of kaempferol were also identified in Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L. extracts, and two glycosides – in the extracts of Borago officinalis L. The conducted study demonstrates the efficiency of the HPLC-MS method for analyzing flavonoids in multicomponent plant matrices and can be used for the quality control of plant raw materials and in pharmacognosy.