Modeling age-related methylation changes in vitro with intestinal organoids

20260 citationsJournal Articlegold Open Access

Authors

Himani Vaidya · Coriell Institute For Medical Research

Gennaro Calendo · Coriell Institute For Medical Research

Kelsey Keith · Children's Hospital of Philadelphia

Anthony Pompetti · Coriell Institute For Medical Research

Curt Balch · Coriell Institute For Medical Research

Jozef Madžo · The Wistar Institute

Jaroslav Jelinek

Abstract

Our findings suggest that DNA methylation changes in organoids reflect two distinct biological processes: aging, as marked by CpG island hypermethylation, and an unexplained stochastic hypomethylation defect. Additionally, treatment with the DNA methylation inhibitor decitabine reduced the methylation age of organoids from 22 to 10 months, demonstrating the potential of organoids as a model for studying extrinsic influences on age-related DNA hypermethylation.

Topics & Keywords

Cancer Cells and Metastasis Epigenetics and DNA Methylation Digestive system and related health

UN Sustainable Development Goals

Zero hunger

Publication Details

Published in: BMC Biology

DOI: 10.1186/s12915-026-02558-y

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