First Report on the Acoustic Signals of Lahille’s Bottlenose Dolphins in Argentina

This study provides the first characterization of the acoustic signals emitted by Lahille's bottlenose dolphins (<i>Tursiops gephyreus</i>) in Argentine waters, establishing a baseline for the species' southern distribution limit. Passive acoustic monitoring was conducted in the inner channels of the Bahía Blanca Estuary using a broadband hydrophone and F-POD detectors. We documented a diverse acoustic repertoire consisting of echolocation clicks, whistles, bray-calls, and chirps. Acoustic presence was predominantly diurnal and showed a marked synchronization with the tidal cycle, with peak activity occurring during the ebbing tide. Whistles had a mean center frequency of 8.1 kHz, showing spectral overlap with other regional populations in the Southwestern Atlantic. Echolocation clicks were broadband, with peak frequencies ranging from 18 to 127 kHz and a mode around 40 kHz. These results suggest that dolphin movement in the estuary's internal channels is structured by hydrodynamics, likely reflecting foraging opportunities associated with tidal flow. Given the endangered status of this species, these data establish a technical framework for non-invasive monitoring and habitat-use assessments in complex estuarine environments.